Merck ABScript II One Step RT-qPCR Probe Kit

ABScript II One Step RT-qPCR Probe Kit

Quality Level

200

사용

sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 500 reactions

특징

dNTPs included
hotstart

technique(s)

DNA amplification: suitable
PCR: suitable
RT-PCR: suitable
RT-qPCR: suitable
cDNA synthesis: suitable
qPCR: suitable

input

purified RNA

호환성

ABI 7000
ABI 7300
ABI 7500
ABI 7700
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad iCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
QuantStudio™
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000

배송 상태

dry ice

저장 온도

−20°C

The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for higher sensitivity. This kit is ideal for analyzing low input RNA samples.The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription of RNA. After reverse transcription, the hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5′-3′ exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal.