Merck JumpStart Taq DNA Polymerase

JumpStart™ Taq DNA Polymerase

without MgCl2

Quality Level

300

form

liquid

사용

sufficient for 1500 reactions
sufficient for 250 reactions
sufficient for 50 reactions

특징

dNTPs included: no
hotstart

농도

2.5 units/μL

technique(s)

PCR: suitable

색상

colorless

input

purified DNA

application(s)

agriculture

배송 상태

wet ice

저장 온도

−20°C

The Taq DNA polymerase activity is inactivated by combining the enzyme with JumpStart™ Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start polymerase chain reaction (PCR). Hot start PCR can significantly improve the results of DNA amplification by reducing the generation of nonspecific amplification products and primer-dimer artifacts. When used in PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active.