Merck PCR Buffer Without MgCl2, 10x concentrated

PCR Buffer Without MgCl2, 10x concentrated

solution, pkg of 3 × 1 mL

buffer

form

solution

Quality Level

100

포장

pkg of 3 × 1 mL

manufacturer/tradename

Roche

배송 상태

dry ice

저장 온도

−20°C

The PCR buffer without MgCl2, in combination with the MgCl2 stock solution, is used for the individual adjustment of the Mg2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl2 stock solution.