Merck Tth DNA Polymerase

Tth DNA Polymerase

pkg of 500 U (2 x 250_U), sufficient for ≤200 reactions

DNA polymerase, polymerase

사용

sufficient for ≤200 reactions

Quality Level

100

포장

pkg of 500 U (2 x 250_U)

manufacturer/tradename

Roche

파라미터

75 °C optimum reaction temp.

최적 pH

~9.0 (25 °C)

배송 상태

dry ice

저장 온도

−20°C

Tth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly processive 5′-3′ DNA polymerase, and lacks 3′-5′ exonuclease activity. The enzyme exhibits its highest activity at a pH of approximately 9 (adjusted at +25°C), and temperatures around +75°C. It is resistant to prolonged incubations at high temperatures (+95°C).