Merck Reverse Transcriptase AMV

Reverse Transcriptase AMV

solution, >50 units/μg protein, suitable for RT-qPCR, suitable for RT-PCR

amv reverse transcriptase

Quality Level

100

form

solution

specific activity

>50 units/μg protein

특징

dNTPs included: no
hotstart: no

포장

pkg of 1,000 U

manufacturer/tradename

Roche

저장 조건

avoid repeated freeze/thaw cycles

파라미터

42 °C optimum reaction temp.

technique(s)

RT-PCR: suitable
RT-qPCR: suitable

input

purified RNA

detection method

probe-based

배송 상태

dry ice

Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are α,ββ and αβ. The molecular weight of the α-subunit is 68 kDa, that of the β-subunit 92 kDa. The mature αβ form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.

Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.