Merck KAPA Taq ReadyMix with dye

KAPA Taq ReadyMix with dye

2 ×, suitable for PCR

Taq, PCR

Quality Level

100

사용

50 μL sufficient for 250 reactions

유통기한

≤18 mo.

특징

dNTPs included
hotstart: no

포장

pkg of 6.25 mL

manufacturer/tradename

Roche

농도

2 ×

technique(s)

PCR: suitable

input

purified DNA

배송 상태

dry ice

저장 온도

−20°C

KAPA Taq DNA Polymerase is a single-subunit Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli. KAPA Taq ReadyMix™ (2X) is a ready-to-use cocktail containing all components for PCR, except primers and template. KAPA Taq ReadyMix is available with and without dye. The 2X ReadyMix with dye consists of two inert tracking dyes to permit direct loading of PCR products onto agarose gels for analysis by electrophoresis, without adding a DNA loading solution. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 5′→3′ polymerase and 5→′3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.