Merck KAPA2G Fast Genotyping Mix

KAPA2G Fast Genotyping Mix

suitable for PCR, 2 ×

genotyping, PCR

Quality Level

100

사용

sufficient for 500 reactions
25 μL sufficient for 500 reactions

유통기한

≤18 mo.

특징

dNTPs included
hotstart: no

manufacturer/tradename

Roche

농도

2 ×

technique(s)

PCR: suitable
genotyping: suitable

input

crude DNA

배송 상태

dry ice

저장 온도

−20°C

KAPA2G Fast Genotyping Mix (2X) is a ready-to-use master mix containing all components for fast polymerase chain reaction (PCR), except primers and template. The ReadyMix™ also contains two inert dyes, allowing for the analysis of PCR reaction products by agarose gel electrophoresis without the need to add a DNA loading solution. The KAPA Express Extract system contains a thermostable protease, designed for rapid sample lysis. The KAPA Express Extract, a novel thermostable protease and buffer system allows the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols.