Merck TRI Reagent

TRI Reagent®

For processing tissues, cells cultured in monolayer or cell pellets

Quality Level

200

사용

mL sufficient for 107 cells
mL sufficient for 100 mg tissue (or)

저장 온도

2-8°C

TRI reagent® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagent® is a mixture of guanidine thiocyanate and phenol in a monophasic solution.
Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.