Merck DNA Isolation Kit for Mammalian Blood

DNA Isolation Kit for Mammalian Blood

kit of for 25 reactions

DNA isolation

Quality Level

100

manufacturer/tradename

Roche

포장

kit of for 25 reactions

Isolation of DNA from whole blood can be difficult because blood is a complex mixture containing cells, proteins, metabolites, etc. Most of the cells (>99%) are erythrocytes (red blood cells) which lack nuclei, and therefore, possess no DNA. Leukocytes (white blood cells) contain nuclei and DNA. Therefore, DNA from blood must be isolated from one of three types of leukocytes: monocytes, lymphocytes, or granulocytes.

Sample material:
Human whole blood (treated with sodium heparin, EDTA or sodium citrate): 1 to 10 mL
Isolated lymphocytes: 1 to 10 mL
Isolated buffy coat: buffy coat from 10 to 20 mL whole blood, containing at least 1.0 x 107 leukocytes.

Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9