Merck GenElute-E Single Spin Tissue DNA Kit

GenElute™-E Single Spin Tissue DNA Kit

정제법

(Single-spin negative chromotography)
(Time: 45 minutes or less)

특징

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For the purificiation of genomic DNA from human or animal tissue)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

환경친화적 대안 카테고리

Aligned

GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers:

• Isolation of DNA in under an hour with minimal sample handling
• Better purity, leading to improved performance in PCR and other applications
• Significant reduction in plastic and hazardous chemical waste

Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.

GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse™ Protease, which enables fast and efficient lysis of a wide range of sample types.

Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.