Merck PCR DIG Labeling MixPLUS

PCR DIG Labeling MixPLUS

sufficient for 2 x 50 assays (50μl final reaction volume), pkg of 2 × 250 μL, solution

form

solution

Quality Level

100

사용

sufficient for 2 x 50 assays (50μl final reaction volume)

포장

pkg of 2 × 250 μL

manufacturer/tradename

Roche

환경친화적 대안 제품 특성

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

환경친화적 대안 카테고리

Aligned

배송 상태

dry ice

저장 온도

−20°C

The PCR DIG Labeling MixPLUS is a mixture of lithium salts of deoxyadenosine triphosphate (dATP), deoxycytosine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), deoxyuridine triphosphate (dUTP) and digoxigenin (DIG)-11-dUTP. This nucleotide mixture can be added directly to polymerase chain reactions (PCR) and the DIG-labeled nucleotide will be incorporated into the PCR product. The incorporation of dUTP instead of deoxythymidine triphosphate (dTTP) allows the degradation of contaminating PCR products from previous amplification reactions with uracil-DNA glycosylase (UNG), preventing carryover contamination.The incorporated DIG label renders the sensitive detection of the PCR product with anti-DIG-conjugates, for example in the PCR ELISA, DIG-labling, and PCR ELISA, DIG-Detection. The PCR DIG Labeling MixPLUS gives comparable signal intensities to the PCR DIG Labeling Mix.