Merck Nick Translation Mix

Nick Translation Mix

sufficient for 50 labeling reactions, pkg of 200 μL, solution

nick translation

form

solution

Quality Level

100

사용

sufficient for 50 labeling reactions

포장

pkg of 200 μL

manufacturer/tradename

Roche

배송 상태

dry ice

저장 온도

−20°C

Optimized enzyme mixture: Nick translation utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix, then incorporates labeled nucleotides as the polymerase examines, or proofreads the nicked site.
Individual templates produce consistent results in the standard 90-minutes reaction, and result in an average probe length of 200 base pairs up to 500 base pairs.
Assay Time: 100 minutes

Sample Materials

• Supercoiled and linearized plasmid DNA
• Supercoiled and linearized cosmid DNA
• Purified PCR products