Merck DIG-Nick Translation Mix

DIG-Nick Translation Mix

sufficient for 40 labeling reactions, pkg of 160 μL, suitable for hybridization

form

solution

Quality Level

100

사용

sufficient for 40 labeling reactions

포장

pkg of 160 μL

manufacturer/tradename

Roche

환경친화적 대안 제품 특성

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

hybridization: suitable

환경친화적 대안 카테고리

Aligned

배송 상태

dry ice

저장 온도

−20°C

DIG-Nick Translation Mix is a convenient enzyme and nucleotide mixture for nick translation. It utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix, then incorporates labeled nucleotides as the polymerase examines, or proofreads the nicked site. Large plasmids, cosmids and polymerase chain reaction (PCR) fragments are all regularly used with this method and the DNA can be linearized or supercoiled. Individual templates produce consistent results in the standard 90-minutes reaction and result in an average probe length of 200 bp up to 500 bp. The molar ratio of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) to deoxythymidine triphosphate (dTTP) is adjusted to ensure that every 20th to 25th nucleotide in the newly synthesized DNA is modified with DIG. This density of haptens in the DNA yields the highest sensitivity in the immunological detection reaction.