Merck PCR DIG Labeling Mix

PCR DIG Labeling Mix

solution, suitable for PCR

form

solution

Quality Level

100

사용

sufficient for 2 x 25 assays (100 ul final reaction volume)

포장

pkg of 500 μL (2 x 250 μl)

manufacturer/tradename

Roche

환경친화적 대안 제품 특성

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

PCR: suitable

환경친화적 대안 카테고리

Aligned

배송 상태

dry ice

저장 온도

−20°C

PCR DIG Labeling Mix is a nucleotide mixture that can be added directly to polymerase chain reactions (PCR) and the digoxigenin (DIG)-labeled nucleotide will be incorporated into the PCR product. Taq DNA polymerase, as well as Tth (Thermus thermophilus) DNA polymerase, can be used for the synthesis of DIG-labeled PCR products. The PCR DIG Labeling Mix can replace the unlabeled nucleotide mix in PCR. 10μl of the PCR DIG Labeling Mix is used in a standard 100μl PCR assay.
When using higher concentrations of DIG-deoxyuridinetriphosphate (dUTP) what is supplied with the PCR DIG Labeling Mix, the yield of the PCR product may be reduced, but however, the label intensity and the molecular weight of the PCR product is increased.