Merck Dansyl labeled polymyxin B Ready Made Solution

Dansyl labeled polymyxin B Ready Made Solution

for fluorescent microbial imaging, 1.5 mg/mL in H2O

DSL-PMB, DSL-PxB, DSL-PMX

Quality Level

100

form

solution

availability

not available in China

농도

1.5 mg/mL in H2O

적합성

suitable for (Fluorescent detection of gram-negative bacteria)
suitable for (Microbiome)
suitable for microbiology

배송 상태

ambient

저장 온도

−20°C

Dansyl Polymyxin B (DSL-PMB) is a fluorescent derivative of Polymyxin B. Its mode of action is in accordance to Polymyxin B activity. However, MIC assays comparing DSL-PMB and parent Polymyxin B showed two to three-fold decrease in bacterial activity.1,2

Polymyxin B is a cationic lipopeptide antibiotic which binds specifically to gram negative bacteria.3-6 It interacts with anionic lipopolysaccharide (LPS) molecules which are located in gram negative bacterial outer membrane.7 This interaction interferes with the outer membrane normal function, causing leakage and enhanced uptake of the antibiotic.7 Resemblance in properties between Polymyxin B and DSL-PMB allows to fluorescently label gram negative bacteria and examine intracellular localization and penetration of Polymyxins in gram negative bacteria.2,8