Merck Lyticase from Arthrobacter luteus

Lyticase from Arthrobacter luteus

free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder

생물학적 소스

bacterial (Arthrobacter luteus)

form

lyophilized powder

specific activity

≥2000 units/mg protein

특징

DNA free

배송 상태

dry ice

저장 온도

−20°C

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.

Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.