Merck CompoZr ZFA Targeting Human OCT4

CompoZr® ZFA Targeting Human OCT4

Quality Level

200

제품 라인

CompoZr®

배송 상태

dry ice

저장 온도

−70°C

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted gene editing. Now, Sigma-Aldrich provides ZF activators, repressors and epigenetic modifiers for gene expression studies. ZFAs, or similarly called ZF−TFs (zinc finger transcription factors) incorporate a transcriptional activation domain in place of the nuclease domain found in ZFNs and specifically activate coding and non-coding genes. The increase in specific gene expression is dependent on the target binding site and the effector domain selected. Native gene activation is through ZFAs binding to promoters and recruiting the transcriptional machinery. This approach for activation also has the benefit of expressing endogenous isoforms at the proper ratio. *Please note all ZFA plasmids are made standard with the p65 activation domain. The end-user will have to swap out the p65 domain and clone in the other activation, epigenetic or repressor domains as needed.