Merck Sigma CRISPR dCAS9-p300V Activator Lenti Plasmid

Sigma CRISPR dCAS9-p300V Activator Lenti Plasmid

Quality Level

200

재조합

expressed in E. coli

form

liquid

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−20°C

This gene activation system is based on a fusion of inactive Cas9 (dCas9) to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300. The dCas9-p300 activator lenti plasmids use the EF1 alpha promoter for strong expression of dCas9-P300 and blasticidin linked by a 2A peptide (EF1a-dCas9-P300-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use Sigma′s lentiviral dCas9-P300 lenti plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing dCas9-P300 for CRISPR based gene activation. The dCas9-P300 lenti plasmid is one part of a two part CRISPR system with individual dCas9-P300 and gRNA expression vectors.

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