Amicon Ultra-2 Centrifugal Filter Unit
Ultracel-50 regenerated cellulose membrane, 2 mL sample volume
Centrifugal concentrator, 50 kDa MWCO, 2 mL sample volume
| Quality Level |
400 |
| material |
polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing |
| 무균 |
non-sterile |
| 제품 라인 |
Amicon® |
| manufacturer/tradename |
Amicon® Ultra |
| 파라미터 |
2 mL sample volume |
| technique(s) |
protein extraction: suitable
protein purification: suitable |
| 길이 |
119.7 mm |
| 직경 |
15.9 mm |
| 여과 면적 |
1 cm2 |
| 필터종류 |
NMWCO |
Amicon® Ultra-2 mL centrifugal devices with Ultracel® 50,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, BSA (67 kDa) at 1 mg/ml initial concentration is typically retained at 97% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
