Amicon Ultra-2 Centrifugal Filter Unit
Ultracel-3 regenerated cellulose membrane, 2 mL sample volume
Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume
| Quality Level |
400 |
| material |
polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing |
| 무균 |
non-sterile |
| 제품 라인 |
Amicon® |
| manufacturer/tradename |
Amicon® Ultra |
| 파라미터 |
2 mL sample volume |
| technique(s) |
protein extraction: suitable
protein purification: suitable |
| 길이 |
119.7 mm |
| 직경 |
15.9 mm |
| 여과 면적 |
1 cm2 |
| 필터종류 |
NMWCO |
Amicon® Ultra-2 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at 100% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
