Merck CRISPR-Lenti Human Positive Control DNA

CRISPR-Lenti Human Positive Control DNA

form

liquid

Quality Level

200

포장

vial of 50 μL

농도

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−20°C

This control is intended for use as a positive control for knockout of human gene targets when used with clones of the Sigma lenti CRISPRs. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).

Validated CRISPR site, which serves as an experimental control for the Sigma lenti CRISPRs. A one-component positive control system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA plasmid targeting the human EMX1 gene, with both Puromycin and GFP co-expressed with Cas9. The Sigma lenti CRISPR Human EMX1 Positive Control Vector is a lentiviral-based plasmid vector that contains a guide RNA sequence targeting the human EMX1 (empty spiracles homeobox 1) gene. EMX1 belongs to the EMX homeobox family. The vector backbone is the same as the Sigma lenti CRISPR products.