Merck SAM CRISPRa Helper Construct Kit Lentiviral Transduction Particles

SAM CRISPRa Helper Construct Kit Lentiviral Transduction Particles

포장

pkg of 16 vials (8x25μL aliquots for each of the 2 kit components)

농도

≥1x106 VP/ml (via p24 assay)

application(s)

CRISPR

배송 상태

dry ice

저장 온도

−70°C

This product is a kit containing two sets of ready to use lentiviral particles enabling immediate transduction for strong expression in a wide range of both dividing and non-dividing cell lines. MS2-P65-HSF1 lentiviral particles efficiently and stably integrate MS2-P65-HSF1 and hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin). dCas9-VP64 lentiviral particles efficiently and stably integrate dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin). They are ideal for avoiding the tedious lentivirus production process and are part of a three part CRISPR system with individual dCas9-VP64. MS2-p65-HSF1 and gRNA expression vectors.

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