Merck MISSION TRC2 pLKO.5-puro TurboGFP shRNA Control Plasmid DNA

MISSION® TRC2 pLKO.5-puro TurboGFP™ shRNA Control Plasmid DNA

shRNA sequence targeting tGFP

MISSION Control Vectors

Quality Level

200

제품 라인

MISSION®

농도

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

배송 상태

dry ice

저장 온도

−20°C

The MISSION® TRC2 Control Vector pLKO-puro TurboGFP™ shRNA is a 7518 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP™. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP™ shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION® TurboGFP™ positive control vector in cell lines expressing TurboGFP™. TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP™ shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.