Merck MISSION TRC2 pLKO.5-puro-CMV-TurboGFP Positive Control Plasmid DNA

MISSION® TRC2 pLKO.5-puro-CMV-TurboGFP™ Positive Control Plasmid DNA

Green fluorescent protein marker to monitor transduction efficiency

MISSION Control Vectors

Quality Level

200

제품 라인

MISSION®

농도

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

배송 상태

dry ice

저장 온도

−20°C

The MISSION TRC2 Control Vector pLKO-puro TurboGFP is an 8802 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the CMV promoter. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP Control Vector is useful as a positive transfection/transduction control in experiments using the TRC2 MISSION shRNA library clones. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.