Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

Target: mmu-miR-297a-5p

Type: Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm employs the Tough Decoy (TuD) design, enabling potent inhibition of target miRNAs.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, which, when co-transfected with compatible packaging plasmids, produces viral particles capable of transducing mammalian cells.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and a puromycin resistance gene for cell selection.

주요 특징

• 강력한 표적 miRNA 억제 효과
• 렌티바이러스 전달 형식으로 다양한 세포에 효율적 적용
• 반복 형질감염 없이 장기적 억제 가능
• 퓨로마이신 내성 유전자를 통한 세포 선택 가능