Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

대상 miRNA

• mmu-miR-202-3p

설계 방식

• Tough Decoy (TuD) 구조 적용
• Haraguchi 등과 도쿄대학교 Hideo Iba 박사 협력 기반의 독자 알고리즘 사용

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design, enabling potent inhibition of the desired miRNA.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, which, upon co-transfection with compatible packaging plasmids, produces viral particles for transduction of mammalian cells.

The vector includes:

• Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression
• Puromycin resistance gene for selection of transduced cells

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 전달 형식으로 다양한 세포 유형에 효율적 전달
• 반복적인 트랜스펙션 없이 장기적 억제 가능