Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-153-5p

Tough Decoy (TuD) Design

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba, University of Tokyo.
This algorithm utilizes the Tough Decoy (TuD) design to effectively inhibit target miRNA activity.
miRNA regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, enabling viral particle production via co-transfection with compatible packaging plasmids.
These viral particles can transduce mammalian cells efficiently, providing stable and long-term inhibition of the desired miRNA.

Additionally, the vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression and a puromycin resistance gene for cell selection.

주요 특징

• Potent inhibition of target miRNA
• Efficient lentiviral delivery across diverse cell types
• Long-term inhibition without repeat transfection
• Puromycin resistance marker for selection

제품 스펙

제품 이미지

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