Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-3094-5p

Design: Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm employs the Tough Decoy (TuD) design to enable potent inhibition of the target miRNA.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation. The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles suitable for transduction of mammalian cells.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression and a puromycin resistance gene for cell selection.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 전달 형식으로 다양한 세포 유형에 효율적 적용 가능
• 반복적인 트랜스펙션 없이 장기적 억제 가능
• 퓨로마이신 내성 유전자 포함으로 선택 용이