Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-3077-3p

Tough Decoy (TuD)

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design for effective inhibition of target miRNAs.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles for mammalian cell transduction.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and carries a puromycin resistance gene for selection.

주요 특징

• 강력한 특정 miRNA 억제 가능
• 렌티바이러스 전달 포맷으로 다양한 세포에 효율적 전달
• 반복 형질감염 없이 장기 억제 구현
• puromycin 저항성 유전자로 세포 선택 용이

제품 사양

참고 문헌

Haraguchi, T. et al., and Dr. Hideo Iba, University of Tokyo.