Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-1933-3p

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al. in collaboration with Dr. Hideo Iba, University of Tokyo.
This algorithm utilizes the tough decoy (TuD) design.

miRNA are known to regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles suitable for transducing mammalian cells.

The Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) is included to enhance transgene expression.
This lentiviral vector also carries a puromycin resistance gene for selection of successfully transduced cells.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 다양한 세포주에 효율적으로 전달 가능한 렌티바이러스 포맷
• 반복적인 트랜스펙션 없이 장기적인 억제 유지 가능