Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-669f-3p

Tough Decoy (TuD)

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba, University of Tokyo.
This algorithm utilizes the Tough Decoy (TuD) design for effective miRNA inhibition.

miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector with compatible packaging plasmids produces viral particles suitable for mammalian cell transduction.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) is included to enhance transgene expression.
This vector also carries a puromycin resistance gene for selection of successfully transduced cells.

주요 특징

• 강력한 miRNA 억제 효과 제공
• Lentiviral delivery로 다양한 세포 유형에 효율적 전달
• 반복 형질감염 없이 장기적인 억제 가능
• 퓨로마이신 내성 유전자를 통한 세포 선택 가능