Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

타깃 microRNA

• mmu-miR-693-3p

디자인

• Tough Decoy (TuD) 기반

품질 등급

• 200

제품 라인

• MISSION®

형태

• liquid

농도

• ≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

• GCAGCUUUCAGAUGUGGCUGUAA

Sanger 성숙/소수 수납 번호

• MIMAT0004189

microRNA 수납 번호

• MI0004662

배송 상태

• dry ice

저장 온도

• −70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba, University of Tokyo.
This algorithm employs the Tough Decoy (TuD) design, enabling strong inhibition of target miRNA activity.
miRNA are known to regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles suitable for transduction into mammalian cells.
The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and a puromycin resistance gene for selection of transduced cells.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 전달 포맷으로 다양한 세포에 효율적 적용
• 반복적인 트랜스펙션 없이 장기적 억제 가능
• WPRE 포함으로 높은 발현 효율 확보
• Puromycin 내성 유전자로 안정적인 세포 선택 가능