Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-5101

Tough Decoy (TuD) 형식

품질 등급

200

제품 라인

MISSION®

형태

Liquid

농도

≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

UUUGUUUGUUUUGCUGAUGCAG

Sanger 성숙/소수 수납 번호

MIMAT0020608

microRNA 수납 번호

MI0018009

배송 상태

Dry ice

저장 온도

−70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design.

miRNA regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles suitable for mammalian cell transduction.

The vector includes Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) for enhanced transgene expression and carries a puromycin resistance gene for cell selection.

주요 특징

• 강력한 목표 miRNA 억제 가능
• Lentiviral 전달 포맷으로 다양한 세포 유형에 효율적 적용
• 반복적인 트랜스펙션 없이 장기적인 억제 유지
• Puromycin 저항성 유전자를 통한 세포 선택 가능