Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-5100

Tough Decoy (TuD) Design

제품 정보

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in various manners, including translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector with compatible packaging plasmids produces viral particles that can transduce mammalian cells.
Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included to enhance transgene expression.
This lentiviral vector also carries a puromycin resistance gene for selection of transduced cells.

주요 특징

• 강력한 miRNA 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포 유형에 효율적 전달
• 반복적인 트랜스펙션 없이 장기적인 억제 가능