Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-18a-3p

Tough Decoy (TuD) Design

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, developed in collaboration with Dr. Hideo Iba (University of Tokyo) and based on the work of Haraguchi et al. This algorithm employs the Tough Decoy (TuD) design to achieve potent and stable inhibition of target miRNA.

miRNAs regulate gene expression via mechanisms such as translational repression, mRNA cleavage, and deadenylation. The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles suitable for transducing mammalian cells.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and a puromycin resistance gene for selection of transduced cells.

주요 특징

• 강력하고 지속적인 miRNA 억제 효과
• 다양한 세포 유형에서 효율적인 렌티바이러스 전달
• 반복 형질감염 없이 장기적 억제 가능
• puromycin 내성 유전자를 통한 세포 선택 가능

제품 사양

참고 문헌

Haraguchi, T. et al., in collaboration with Dr. Hideo Iba, University of Tokyo.