Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

타깃 microRNA

• mmu-miR-151-5p

디자인 방식

• Tough Decoy (TuD) 기반 설계

제품 라인

• MISSION®

품질 등급

• 200 (M-Clarity Program)

제품 형태 및 농도

서열 정보

배송 및 보관

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent and specific inhibition of target miRNA.

miRNAs regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector, which enables co-transfection with packaging plasmids to produce viral particles suitable for mammalian cell transduction.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and a puromycin resistance gene for selection of successfully transduced cells.

주요 특징

• 강력한 miRNA 억제 효능
• 렌티바이러스 전달 포맷으로 다양한 세포에 효율적 전달
• 반복적인 트랜스펙션 없이 장기적 억제 가능
• puromycin 저항성 유전자로 안정적인 세포 선택 가능