Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-1251-5p

Tough Decoy (TuD)

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design.
miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles for mammalian cell transduction.
The vector includes WPRE (Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2) for enhanced transgene expression and a puromycin resistance gene for cell selection.

주요 특징

• 원하는 miRNA의 강력한 억제 가능
• 렌티바이러스 전달 형식으로 다양한 세포에 효율적 전달
• 반복 형질감염 없이 장기적 억제 가능
• 퓨로마이신 내성 유전자를 통한 세포 선택 가능

제품 사양

참고 문헌

Haraguchi, T. et al., and Dr. Hideo Iba, University of Tokyo — development of the proprietary TuD design algorithm.