Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

대상 miRNA

• mmu-miR-486-3p

디자인

• Tough Decoy (TuD) 방식

품질 등급

• 200

제품 라인

• MISSION®

제형

• Liquid

농도

• ≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

• CGGGGCAGCUCAGUACAGGAU

Sanger 성숙/소수 수납 번호

• MIMAT0017206

microRNA 수납 번호

• MI0003493

배송 상태

• Dry ice

저장 온도

• −70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent inhibition of target miRNA.

miRNA regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids produces viral particles suitable for transduction into mammalian cells.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced transgene expression and a puromycin resistance gene for cell selection.

주요 특징

• 강력한 miRNA 억제 효과
• 렌티바이러스 전달 방식으로 다양한 세포에 효율적 도입 가능
• 반복적인 트랜스펙션 없이 장기적 억제 유지