Merck MISSION Lenti microRNA, Mouse

MISSION® Lenti microRNA, Mouse

mmu-miR-450a-5p

제품 개요

Sigma의 MISSION® Lenti-miRs는 공통 백본 구조를 기반으로 miRNA를 발현하며, 정확한 Dicer 처리를 위해 설계되었습니다. 부분 상보적 가닥은 독자 알고리즘을 통해 백본 구조의 염기쌍 패턴을 모방하도록 디자인되었습니다.

Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase. Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors.

Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allows for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.

Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.

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