Merck MISSION Lenti microRNA, Human

MISSION® Lenti microRNA, Human

hsa-miR-372-3p

제품 개요

Sigma의 MISSION® Lenti-miRs는 정확한 Dicer 처리를 위한 공통 백본 구조를 기반으로 설계된 인간 microRNA 발현용 렌티바이러스 입자입니다.
부분 상보 서열은 독자 알고리즘을 통해 백본 구조의 염기쌍 패턴을 모방하도록 설계되었습니다.
각 miRNA 구성체는 TRC2-pLKO-puro 벡터에 클로닝되고, 시퀀스 검증을 거쳐 생산됩니다.

제품 사양

제품 설명

Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors.
Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector.
Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells.

The polymerase II promoter, elongation factor 1 alpha (EF1A), drives miRNA expression required for reverse transcription of viral RNA and integration of viral DNA into the host genome.
Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) enhances transgene expression delivered by lentiviral vectors.

This vector also includes a puromycin resistance gene for selection of transduced cells.
Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles allow efficient infection and integration into both dividing and non-dividing cells, such as neurons and dendritic cells.