Merck MISSION Lenti microRNA, Mouse

MISSION® Lenti microRNA, Mouse

mmu-miR-432

제품 개요

Merck의 MISSION Lenti-miRs는 공통 백본 구조를 기반으로 microRNA를 발현하도록 설계된 렌티바이러스 입자입니다.
정확한 Dicer 처리 요구사항을 충족하며, 상보적 가닥은 독자적 알고리즘을 통해 백본 구조 내 염기쌍 패턴을 모방하도록 설계되었습니다.
microRNA 서열을 포함하는 올리고는 TRC2-pLKO-puro 벡터에 클로닝되며, 각 miRNA 구조체는 서열 검증을 거칩니다.

제품 사양

제품 설명

• Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors.
• Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector.
• Co-transfection into the appropriate cell line with compatible packaging plasmids produces viral particles for mammalian cell transduction.
• The EF1A polymerase II promoter drives miRNA expression, facilitating reverse transcription and integration into the host genome.
• The Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) enhances transgene expression.
• The vector includes a puromycin resistance gene for selection.
• Lentiviral-based systems enable efficient infection and integration into both dividing and non-dividing cells, such as neurons and dendritic cells, unlike MMLV or MSCV retroviral systems.