Merck MISSION pLKO.1-puro Luciferase shRNA Control Transduction Particles

MISSION® pLKO.1-puro Luciferase shRNA Control Transduction Particles

shRNA sequence targeting luciferase

MISSION TurboGFP Control Transduction Particles

Quality Level

200

제품 라인

MISSION®

농도

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

배송 상태

dry ice

저장 온도

−70°C

The MISSION Luciferase shRNA Control Transduction Particles contain an shRNA sequence targeting Luciferase, Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing firefly luciferase.
The Luciferase shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-luciferase shRNA (Prod. No. SHC007). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.