Merck MISSION pLKO.1-puro Luciferase shRNA Control Plasmid DNA

MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA

shRNA sequence targeting luciferase

MISSION Control Vectors

Quality Level

200

제품 라인

MISSION®

농도

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

배송 상태

dry ice

저장 온도

−20°C

The MISSION® Luciferase shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting luciferase from Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing firefly luciferase. It can also be used as a negative control vector.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids, MISSION® Lentiviral Packaging Mix (Prod. No. SHP001). The Luciferase shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.