Merck Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit

Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit

100 assays in 96 well plates

사용

100 assays in 96 well plates

저장 조건

protect from light

NCBI 수납 번호

NM_001122765 (Mouse)
NM_001193286 (Human)

배송 상태

wet ice

저장 온도

−20°C

Gene Information

human ... SIRT2(22933)
mouse ... SIRT2(64383)

Sirtuin 2 (SIRT2) is a cytoplasmic protein and is part of the sirtuin family of proteins. Sirtuin family contains a sirtuin core domain and isgrouped into four classes with SIRT2 being a member of class I. SIRT2 colocalizes with microtubules. The gene encoding this protein is localized on human chromosome 19q13.2.

With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.