Merck Histone Deacetylase 8 (HDAC8) Activity Assay Kit

Histone Deacetylase 8 (HDAC8) Activity Assay Kit

100 assays in 96 well plates

Quality Level

100

사용

100 assays in 96 well plates

NCBI 수납 번호

NM_001166418 (Human)
NM_027382 (Mouse)

배송 상태

wet ice

저장 온도

−20°C

Gene Information

human ... HDAC8(55869)
mouse ... HDAC8(70315)

Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.

With Sigma′s HDAC8 Activity Assay Kit, HDAC8 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC8 Substrate (R-H-K(Ac)-K(Ac)-AFC). This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC8 activity. The kit provides a rapid, simple, sensitive, and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC8, and from native, recombinant, or genetically modified HDAC8.