Merck EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit

EZ-Magna ChIP® A/G Chromatin Immunoprecipitation Kit

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A/G beads. Control primers included.

Magnetic ChIP Kit

Quality Level

100

manufacturer/tradename

Magna ChIP®

technique(s)

immunoprecipitation (IP): suitable

배송 상태

dry ice

Unlike standard ChIP protocols that can be laborious and time consuming, the Magna ChIP kit protocol can reduce the amount of time required to perform a ChIP experiment from three days to one. Additionally, the smaller Magna ChIP reaction volume increases the relative concentration of the antibody enabling the ChIP reaction to be performed with reduced amounts of both antibody and sheared chromatin. Finally because this kit uses a blend of protein A and protein G beads, a wider range of antibody isotypes can be used than A or G alone. This allows a wider variety of antibodies to be used and avoids the need to purchase separate kits for protein A and protein G based immunoprecipitation. Because Magna ChIP kits use paramagnetic beads they are compatible with automated high throughput platforms, thus allowing a large number of ChIP reactions to be carried out simultaneously. Features & Benefits:

• Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
• Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
• Negative and positive control antibodies and control primer set to simplify validation of experimental procedure
• Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
• Complete kit with all required reagents for reliable and reproducible results
• Compatible with ChIPAb+ validated antibody and primer sets

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.