Merck DOTAP Liposomal Transfection Reagent

DOTAP Liposomal Transfection Reagent

>99% (TLC), liquid, suitable for transfection

N-(2,3-Dioleoyloxy-1-propyl)trimethylammonium methyl sulfate, DOTAP methosulfate, Transfection reagent, liposomal transfection reagent, dotap, transfection reagent, dotap

Quality Level

100

설명

N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate

assay

>99% (TLC)

form

liquid

포장

pkg of 5 × 400 μL (10 U/μl)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

배송 상태

wet ice

저장 온도

2-8°C

SMILES string

[H]C(C[N+](C)(C)C)(OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O.O=S([O-])(OC)=O

InChI

1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1/b22-20-,23-21-;

InChI key

RSMRWWHFJMENJH-LQDDAWAPSA-M

DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods.