Merck PSF-CMV-FMDV-NEO/G418 - FMDV IRES G418 EXPRESSION PLASMID

PSF-CMV-FMDV-NEO/G418 - FMDV IRES G418 EXPRESSION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 3917 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This vector allows the expression of two genes from one vector where the second gene produced from the mRNA is the neomycin (G418) (G418) antibiotic resistance gene (Neo) under the control of the internal ribosome entry site (IRES) from Encephalomyocarditis virus (ECMV). Transcription is driven by the CMV promoter. There is a multiple cloning site immediately downstream of the CMV promoter which is then followed by the IRES element driving the Neo gene.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.