Merck PSF-CMV-DELTA-D78NCOI - START CODON (NCOI) DELETED PLASMID

PSF-CMV-DELTA-D78NCOI - START CODON (NCOI) DELETED PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4304 bp

박테리아 선정

ampicillin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid yields high levels of protein expression from the CMV promoter in mammalian cells but it contains no start codon in the MCS unlike most of our other plasmids. This allows you to insert genes into the downstream restriction sites and use your own genes start codon if required thereby preventing premature translation initiation at the start codon that was within the NcoI site.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.