Merck PSF-CMV-UB-NEO/G418 ASCI - G418 SELECTION PLASMID

PSF-CMV-UB-NEO/G418 ASCI - G418 SELECTION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4256 bp

박테리아 선정

kanamycin

포유류 세포 선정

neomycin / G418

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This vector contains a Neomycin (Neo)/G418 resistance expression cassette under control of the Ubiquitin (Ub) promoter that is designed as an add-on to any of our vectors. The entire Ub Neo/G418 cassette is flanked by AscI sites. This vector can be used to force recombination into the genome of mammalian cells following transfection and selection using G418 although this method can be inefficient. It can also be used conjunction with the SV40 origin of replication in cells expressing the SV40 large T antigen (such as 293T cells) to make stable cells lines following transfection and selection.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.